Changes In Bone Mass, Bone Turnover Indicators, And Estrogen Levels In Rats After Glucocorticoid Intervention And Their Relationships
Keywords
Glucocorticoid Osteoporosis, Bone Mass, Type I Procollagen Amino-Terminal Propeptide, Type I Collagen Carboxy-Telopeptide, Estrogen, Rat
Abstract
Objective To explore the changes in bone mass, bone turnover indicators, and estrogen levels in rats at different time points after glucocorticoid (GC) intervention and their relationships. Methods Thirty-four 3-month-old female SD rats with body weight (208.73¡À23.94) g were randomly divided into baseline group (n=6), control group (n=14), and dexamethasone (DXM) group ( n=14). The DXM group was injected subcutaneously with DXM 0.75 mg/kg, twice a week for 12 consecutive weeks; the control group was injected with the same amount of normal saline; the rats in the baseline group were not treated. Rats were taken from the baseline group before the experiment, the control group and the DXM group at 4, 8, and 12 weeks to measure body weight, uterus, and adrenal gland weight; the serum level of type I procollagen amino-terminal propeptide (N-terminal propeptide of type I procollagen (PINP), C-terminal cross-linking telopeptide of type I collagen (¦Â-CTX) and estrogen levels; dual-energy X-ray absorptiometry was used to scan the L1-3 vertebrae and For the left femur, measure the bone mineral density (BMD) and bone mineral content (BMC) of the femoral head and neck, femoral tuberosity, femoral shaft, entire femoral segment, and L1, L2, L3, and L1-3 regions. , bone area (bone area, BA). Correlation analysis was performed on the body weight, lumbar spine BMD, femoral BMD, PINP, ¦Â-CTX, and estrogen levels of rats. Results At each time point, the body weight, uterine and adrenal gland weights of rats in the DXM group were significantly lower than those of the control group and baseline group (P<0.05). The serum PINP and ¦Â-CTX of rats in the DXM group increased after hormone intervention, but there was no statistically significant difference compared with the baseline group and the control group at 4 and 8 weeks (P>0.05), and the difference was statistically significant at 12 weeks (P <0.05); the serum estrogen levels of rats decreased, and were significantly lower than those of the baseline group and the control group at each time point (P<0.05). The BMD, BMC, and BA of the DXM group at each time point were significantly lower than those of the control group (P<0.05), with the most severe bone loss in L2 and femoral trochanter. At 4 weeks, L1 BMC, BA and femoral shaft BA were significantly lower than those in the baseline group (P£¼0.05), while the BMD, BMC, and BA in other parts of the body were not significantly different from the baseline group at each time point (P£¾0.05). ). Correlation analysis showed that lumbar spine and femoral BMD were negatively correlated with PINP and ¦Â-CTX (P<0.05), and positively correlated with estrogen levels (P<0.05). Conclusion After GC intervention, the lumbar vertebrae and femoral bone mass of rats showed a trend of first rapidly decreasing and then maintaining low levels. The bone turnover indexes continued to increase after hormone intervention. The serum estrogen levels showed an overall downward trend. Body weight, bone turnover indexes and Estrogen levels are closely related to bone mass For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Tang Jingjing, Yao Zhensong, Cui Jiancao, Zhuang Hong, Zhang Shuncong, Yang Zhidong, Lin Shunxin, Liang De, Jiang Xiaobing, Shen Gengyang
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