Changes In Scleral Tissue Structure And Biomechanical Properties During Postembryonic Development Of Sclera
Keywords
Sclera,Collagen,Fibrils,Fibroblasts,Elastic,Modulus
Abstract
Objective To explore the changes in morphological structure and biomechanical properties of rabbit scleral tissue at different ages. Methods: New Zealand white rabbits aged 1, 2, and 3 months were selected, the eyeballs were removed, and the diameter and axial length of the eyeballs were measured. The scleral tissue was obtained, and the thickness of the scleral tissue was measured. After a part of the scleral tissue was fixed, HE staining was performed to observe the scleral structure. After a part of the scleral tissue was fixed, the scleral structure was observed. The collagen fibrils were observed through an electron microscope, and another part of the scleral tissue was made into a scleral strip, and the elastic modulus of the sclera was measured using an Instron 5544 testing machine. Results: Eyeball diameter, axial length and scleral thickness increased with age. The elastic modulus of scleral tissue increases with age. The number of scleral fibroblasts in the scleral tissue decreases with age, and the fiber bundles increase and become thicker. The diameter of collagen fibrils increases with age. Conclusion During the postembryonic growth of sclera, the structures of the eyeball and sclera continue to change, collagen fibers continue to increase and thicken, and the biomechanical properties of scleral tissue also improve. Understanding the mechanism of postembryonic growth and development of sclera can provide guidance for the treatment of scleral-related diseases. Provide theoretical guidance for prevention and treatment. Objective To investigate the changes of morphological structures and biomechanical properties of scleral tissues in rabbits at different month ages. Methods The eyeballs of 1, 2 and 3 month-old New Zealand white rabbits were obtained for measuring the diameter and axial length, and the thickness of scleral tissues. Part of the scleral tissues was used to observe scleral structures with HE staining, some other part was used to observe collagen fibrils by electron microscope, and the left part were cut into strips and used to test the elastic modulus of the sclera on Instron 5544 system. Results The diameter, axial length of eyeballs and the thickness of the sclera were increased with month age. The elastic modulus of scleral tissues was also increased with month age. The numbers of scleral fibroblasts were decreased and the numbers of fiber bundles were increased with month age. The diameters of collagen fibrils were increased with month age. Conclusions In the post-embryonic stages, the structures of eyeball and sclera are changed continually, with growing numbers of thicker collagen fibers, and the biomechanical properties of scleral tissues are improved correspondingly. The mechanism of post-embryonic development in sclera is further explained in the study, which can provide theoretical guidance for prevention of sclera-related diseases.
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Original research was done by Hao Shuang, Deng Aijun, Wang Guohui
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