Cyclosporine A Inhibits Vasopressin-Induced Cardiac Fibroblast Proliferation And Collagen Synthesis
Keywords
Abstract
Objective To explore the effect of cyclosporine A (CSA) on the proliferation and collagen synthesis of cardiac fibroblasts (CFS) induced by vasopressin (AVP). Methods: Neonatal Sprague-Dawley rat CFs were cultured using trypsin digestion method. Cells cultured in serum-free medium for more than 24 hours were given different concentrations of CSA based on AVP stimulation. The cell number was determined by the mtt method, the cell cycle was analyzed by flow cytometry, the hydroxyproline content of the cell culture supernatant was determined by the hydroxyproline colorimetric method, and the calcineurin (can) activity was measured by a spectrophotometer. Results Cyclosporine A dose-dependently inhibited the increase in the number of cardiac fibroblasts induced by AVP. The inhibition rates of 0.05, 0.5 and 5 ¦Ìmol¡¤l-1 CSA on the MTT absorbance value of cfs were 12%, 24% and 24% respectively. 29% (both p<0.05); cell cycle analysis showed that 0.5¦Ìmol¡¤l-1csa could reduce the S phase percentage and proliferation index induced by avp (p<0.05); 0.5 and 5¦Ìmol¡¤l-1csa could reduce the cell culture supernatant Liquid hydroxyproline content, inhibition rates were 29% and 33% respectively, with statistical differences (all p<0.05); csa can completely block avp-induced can activity in cfs cells, while csa has a negative effect on can activity in the basal state and cell viability were not significantly affected. Conclusion CSA inhibits AVP-induced CFS proliferation and collagen synthesis by blocking the CAN signaling pathway, inhibits the process of myocardial fibrosis, and provides a new therapeutic target for the prevention and treatment of myocardial fibrosis. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Shang Fujun, Zhao Lianyou, Zheng Qiangsun, Wang Jiepin
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