Effect Of Alendronate Saium And Collagen Type I Complex Films Of Nano-Hydroxyapatite Surface On Osteoblasts Morphology And Proliferation.
Keywords
Nano-Hydroxyapatite, Alendronate Sodium, Collagen Type I, Osteoblast, Nano-Hydroxyapatite, Alendronate Saium, Collagen Type I, Osteoblast
Abstract
Abstract Purpose: To explore the effect of nano-hydroxyapatite (nHA) coating modified by chemical combination of alendronate sodium (ALN) and type I collagen (COL I) on osteogenesis Effects on cell adhesion, growth and proliferation on their surfaces. Methods: nHA coating, nHA/ALN, nHA/COL I, nHA/ALN/COL I and nHA/COL I/ALN composite membranes were established, osteoblasts were seeded on the surface of the composite membranes, cultured for 7 d and 14 d respectively, and scanned The changes in the adhesion morphology of osteoblasts on the surface of different membrane layers were observed under an electron microscope, and the number of cells on the surface of each membrane layer was quantitatively detected by WST-1 method. Results: The proliferation activity of osteoblasts was the highest on the surface of nHA/ALN/COL I and nHA/COL I/ALN composite membranes (P<0.05). Scanning electron microscopy observed that osteoblasts had the highest proliferation activity on the surfaces of nHA/ALN/COL I and nHA/COL I. The growth status of the surface of the /ALN composite membrane was better than that of other membrane layers. Under the direct action of the COL I membrane, the osteoblasts stretched more completely, and had more pseudopod protrusions and microvilli structures showing a differentiated phenotype. Conclusion: Modification of ALN and COL I on the surface of nHA can synergistically promote the adhesion and proliferation of osteoblasts. nHA/ALN/COL I composite membrane promotes osteoblast adhesion and differentiation For further information of this article and research, feel free to contact our team for asssitance. Original research was done by JIANG Yan, YAO Jiang-Wu
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