Effect Of Collagen Enzymatic Hydrolyzates On The Properties Of Dialdehyde Starch-Gelatin Composite Films
Keywords
Collagen Hydrolysate Tilapia Skin Gelatin Film Dialdehyde Starch Cell Viability Keratinocytes Collagen Hydrolysate Tilapia Skin Gelatin Film Dialdehyde Starch Cell Viability Keratinocytes
Abstract
This article investigated the effect of tilapia skin collagen enzymatic hydrolyzate (TSCH) on the physical and chemical properties of dialdehyde starch (DAS) cross-linked gelatin membrane, and explored the compatibility of the membrane with human skin immortalized keratinocytes (HaCat). The results showed that when the amount of TSCH added increased from 0 to 16%, the tensile strength of the DAS cross-linked gelatin film decreased from 55.00 MPa to 24.85 MPa, while the elongation at break first increased and then decreased with the increase in the amount of TSCH added. the trend of. The dissolution rate of DAS cross-linked gelatin film in 30¡ãC water is 33.10%. When the TSCH content in the film exceeds 4%, the dissolution rate of the film is close to 100%. However, regardless of the amount of TSCH added, all the TSCH contained in the membrane was released into the water after 240 min. The results of SDS-PAGE showed that the addition of TSCH in the DAS cross-linked gelatin film would hinder the mutual cross-linking of gelatin protein molecules. On the other hand, when HaCat cells were cultured on DAS cross-linked gelatin membrane, the viability of HaCat cells was only 11.39%. When the DAS cross-linked gelatin membrane contained 8% TSCH, the viability of HaCat cells could reach 108.20%. The above results show that the DAS cross-linked gelatin film added with TSCH will have certain application potential in the field of cosmetics. The effect of tilapia skin collagen hydrolysate (TSCH) on the physicochemical properties of dialdehyde starch (DAS) cross-linked gelatin films was investigated, and the biocompatibility of these films with the human skin keratinocyte (HaCat) cell was also studied. When the TSCH content in the DAS-gelatin films was increased from 0 to 16%, the tensile strength (TS) of films decreased from 55.00 MPa to 24.85 MPa, while the elongation at break (EAB) showed a downward trend after the initial increase with increasing amount of added TSCH. The solubility of DAS cross-linked gelatin films in water at 30 ¡æ was 33.10%, and at TSCH concentration higher than four percent, it increased to 100%. However, TSCH from films was released into water after 240 min irrespective of the amount of added TSCH. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the addition of TSCH in the DAS cross-linked gelatin film could hinder the interaction between the protein molecules of gelatin. On the other hand, when HaCat cell was cultured on the surface of DAS cross-linked gelatin films, the cell activity of HaCat cells was 11.39%. When the DAS cross-linked gelatin film contained 8% TSCH, the HaCat cell activity reached up to 108.2% . Based on the above results, the DAS-gelatin films containing TSCH have potential applications in the cosmetic industry.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Chen Shulin
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
