Effect Of Shrna Interfering With Fap Protein Expression On The Growth Of Lewis Lung Cancer In Mice
Keywords
Shrna Fibroblast Activation Protein Lung Tumor Microvessel Density Collagen
Abstract
Abstract: [Objective] To study the effect of shRNA interfering with the expression of fibroblast activation protein (FAP) on the growth of Lewis lung cancer in mice and explore the possible mechanism. [Method] C57 female mice were established with subcutaneous transplantation tumor and lung metastasis models, and were randomly divided into 3 groups: FAP-shRNA group, HK group, and 5% glucose (5% GS) group, which received FAP-shRNA interference plasmid liposome complex respectively. Treatment with substance, empty plasmid liposome complex and 5% glucose water. The size and weight of the transplanted tumors, the number of lung metastases and the wet weight of the lungs were recorded. The FAP expression level, tumor microvessel density, and collagen fiber status in each group were detected. [Results] The growth of subcutaneous transplanted tumors in mice in the FAP-shRNA group was slower than that in the 5% GS group and HK group. The number of lung metastasis nodules and lung wet weight in the FAP-shRNA group were both smaller than those in the 5% GS group and HK group. In addition, in the FAP-shRNA group, the expression of FAP was down-regulated, the density of tumor microvessels was reduced, the expression of type I collagen was increased, and the deposition of collagen fibers was increased and their distribution was disordered. [Conclusion] The anti-tumor effect of FAP-shRNA interference plasmid may be achieved by reducing tumor angiogenesis, increasing type I collagen expression, and ultimately changing the tumor microenvironment.
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Original research was done by 10.11735/j.issn.1671-170X.2014.10.B001
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