Effects Of Bee Venom On Trka And Trpv1 In Dorsal Root Ganglia In Rats With Collagen-Induced Arthritis Inflammatory Pain
Keywords
Bee Venom, Capsaicin Receptor, Tyrosine Kinase A, Rheumatoid Arthritis, Collagen-Induced Arthritis
Abstract
Objective To explore the effect of bee venom on TrkA and TRPV1 pain signaling molecules in rats with collagen-induced arthritis (CIA). Methods: The subjects were divided into normal control group, model group and bee venom group (BV, 3 mg/mL). Wistar male adult rats and Collagen ¢ò+IFA 0.2 mL were used to create the model. In the BV group, medication was started 14 days after modeling and continued until 21 days; the plantar thickness, pain threshold and swollen joint scores of each group were recorded. IHC was used to observe the expression of TRPV1 in pathological sections of the dorsal root ganglion and western bolting was used to observe the expression of TrkA. To evaluate the intervention of bee venom on the ankle joint of CIA rats. Results: The swelling started on the 10th day after the modeling, and all signs of CIA appeared within 14 days. The thickness of the sole of the foot and the swollen joint score showed an overall gradually increasing trend. It levels off at 14 days. The BV group was treated with 0.3 mg of bee venom for 7 days. The thickness of the plantar and swollen joint scores of the BV group were lower than those of the model group. Pain threshold (seconds): normal control group 15.47¡À0.35, model group 10.90¡À0.10, BV group 13.14¡À0.18. Immunohistochemistry percentage of TRPV1-positive cells (%): 11.40¡À1.48 in the normal control group, 44.47¡À4.38 in the model group, and 21.60¡À2.20 in the BV group. Western blotting was used to observe TrkA expression (gray value): 1.59¡À0.04 in the normal control group, 4.53¡À0.21 in the model group, and 2.46¡À0.17 in the BV group. Conclusion Bee venom injection can reduce the expression levels of DRG signaling molecules TrkA and TRPV1 in rats with inflammatory pain. This may be one of the anti-inflammatory and analgesic signaling pathways, providing new inspiration for the treatment of rheumatoid arthritis with bee venom.
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