Effects Of Electrical Stimulation At Different Frequencies On Metabolism Of Collagen In Pelvic Supportive Tissue In Mice With Stress Urinary Incontinence Model
Keywords
Electrical Stimulation, Vaginal Dilation, Stress Urinary Incontinence, Urodynamics, Collagen
Abstract
Objective: To explore the effect of electrical stimulation on collagen metabolism of pelvic floor tissue in mice with stress urinary incontinence (SUI) model induced by vaginal dilatation (VD). Methods: After 20Hz or 50Hz frequency electrical stimulation (ES) was applied to SUI model mice for 7 days, urodynamic indicators were measured to evaluate the modeling effect, and immunofluorescence double-labeling method and Western-blot method were used to detect the pelvic floor tissue of mice before and after electrical stimulation ¢ñ Changes in the content of type collagen (Col¢ñ) and type ¢ó collagen (Col¢ó). Results: Compared with the control group, the maximum bladder capacity (MBC) and leakage point pressures (LPPs) of mice in the VD group were significantly increased (P<0.001), and the relative fluorescence values and protein expressions of Col¢ñ and Col¢ó were significantly decreased (P< 0.001), and the collagen I/III ratio decreased, and the collagen morphology was loose and disordered; compared with the VD group, the MBC and LPPs values of mice after VD+ES treatment were significantly increased (P<0.01, P<0.001), and the relative fluorescence values of Col¢ñ and Col¢ó There was a significant increase (P<0.05, P<0.01), and the I/III collagen ratio increased (P<0.01, P<0.001). Among them, the increase in relative fluorescence value and protein expression in the VD+ES50 group (P<0.01) was higher than that in the VD+ES20 group. group (P<0.05). Conclusion: Electrical stimulation treatment can effectively improve the bladder urinary storage function and urethral continence function of SUI model mice. The effect of high-frequency electric stimulation at 50 Hz is more significant. The mechanism may be related to the promotion of type I and III collagen in the mouse pelvic floor tissue. Metabolism related For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Yaodan, Hong Li, Li Yang, Li Suting, Wang Tingting
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