Effects Of Goose Bone Collagen And Peptides On The Proliferation And Osteogenic Differentiation Of Rat Bone Marrow Mesenchymal Stem Cells
Keywords
Goose Bone Collagen Goose Bone Collagen Peptides Bone Marrow Mesenchymal Stem Cells Proliferation And Differentiation Goose Bone Collagen Goose Bone Collagen Peptides Bone Marrow Mesenchymal Stem Cells Proliferation Differentiation
Abstract
This experiment studied the effects of goose bone collagen, calcium-containing collagen, collagen peptides, and calcium-containing collagen peptides on the proliferation and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into osteoblasts (OB). The effect of goose bone collagen and peptides on osteoporosis is explained. The MTT (thiazole blue) method was used to determine the effect of goose bone collagen and peptides on the proliferation of BMSCs; alkaline phosphatase (ALP) staining, ALP activity determination, alizarin red staining and osteocalcin (BGP) secretion determination Analyze the osteogenic differentiation ability of BMSCs. The results showed that compared with the control group, the experimental group could promote the proliferation of BMSCs. On the 14th day of ALP staining, the positive rate of ALP staining in the experimental group was significantly higher than that of the control group (p<0.05). On the 7th and 14th day, the levels of goose bone collagen and The peptide can significantly increase the activity expression of ALP (p<0.05). On the 21st day, the number of calcium nodules in the alizarin red staining test group was significantly increased compared with the control group (p<0.05), and the osteocalcin secretion in the test group was significantly higher. Compared with the control group (p<0.05). This shows that macromolecular collagen, calcium-containing macromolecular collagen, small molecule collagen peptides, and calcium-containing small molecule collagen peptides can all promote the proliferation and differentiation of BMSCs into osteoblasts, and calcium-containing small molecule collagen peptides have the greatest effect. good. The effects of collagen, collagen containing calcium, collagen peptides, and collagen peptides containing calcium extracted from goose bones on the proliferation of rat bone marrow mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts (OBs) were studied, and the mechanism by which collagen and peptides influence osteoporosis was described. The effects of goose bone collagen and peptides on BMSC proliferation were determined by an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The osteogenic differentiation capacity of BMSCs was measured by alkaline phosphatase (ALP) staining, ALP activity, Alizarin Red staining, and osteocalcin secretion. The experimental groups exhibited greater proliferation of BMSCs than the control group. The positive ALP staining rate of the experimental group on day 14 ALP activity levels of the experimental group on days 7 and 14 were significantly higher than that of the control group (p < 0.05). On day 21, the number of calcified nodules of the experimental groups, detected by Alizarin Red staining, was significantly higher than that of the control group (p < 0.05). The osteocalcin secretion of experimental groups was significantly higher than that of the control group. These results indicated that macromolecular collagen, macromolecular collagen containing calcium, small collagen peptides, and small collagen peptides containing calcium promote BMSC proliferation and the differentiation of BMSCs into OBs, and the small collagen peptides containing calcium displayed the strongest effect. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Jiang Jinli
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