Effects Of Il-27 And Its Receptors On Proliferation And Transformation Of Lung Fibroblasts
Keywords
Interleukin 27 Receptor, Proliferation, Transformation, Lung Fibroblasts, Collagen Synthesis
Abstract
Objective To explore the effects of IL-27 and its receptor (wsx-1) on the proliferation, transformation and collagen synthesis of mouse lung fibroblasts induced by TGF-¦Â1. Methods Construction of il-27r/pcdna3.1 overexpression vector. The experiment was divided into 6 groups: normal group, tgf-¦Â1 group, il-27 group, il-27 receptor group, il-27+il-27 receptor group, tgf-¦Â1+il-27+il-27 receptor group body group. The cell growth status was observed under a light microscope, and mtt was used to detect the effect of each treatment group on the proliferation of lung fibroblasts. RT-PCR and western blotting were used to detect the expression of myofibroblast marker a-SMA and types I and III collagen, and immunofluorescence was used to detect the localization and expression of a-SMA protein. Results (1) Both il-27 and il-27r can inhibit the proliferation of lung fibroblasts; (2) tgf-¦Â1 promotes the increase of ¦Á-sma expression in lung fibroblasts and promotes the synthesis of type I and III collagen in lung fibroblasts; (3) il-27 and its receptor can inhibit the expression of ¦Á-sma in lung fibroblasts and reduce the synthesis of type I and III collagen in lung fibroblasts; (4) When il-27/il-27r work together, It has no effect on the proliferation and transformation of lung fibroblasts. Conclusion il-27 or il-27 receptor can inhibit the proliferation, transformation and collagen synthesis of mouse pulmonary fibrosis cells induced by tgf-¦Â1, but it cannot function in the coexistence of il-27 and il-27 receptor. Inhibitory effect, which may be due to the inability of exogenous IL-27 to neutralize IL-27 receptors to activate cell-related signaling.
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Original research was done by Dong Zhaoxing, Tai Wenlin, Lei Wen, Wang Ying, Li Zhenkun, Zhang Tao
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