Effects Of Mir-144 Overexpression Lentivirus On The Pathological Progression Of Synovial Tissue In Arthritis Model Mice
Keywords
Microrna-, Rheumatoid Arthritis, Cadherin, Tumor Necrosis Factor-¦¡, Type Ii Collagen
Abstract
Objective To explore the effect of miR-144 overexpression lentivirus on the pathological process of synovial tissue in arthritis model mice and its possible mechanism. Methods: Type II collagen emulsion was injected subcutaneously into the tail base of BALB/c mice to construct a mouse model of collagen-induced arthritis (CIA), and then a lentivirus with high expression of miRNA-144 was injected into the tail vein. Then, immunohistochemistry was performed on the synovial tissue of the model mice to detect the expression and distribution of Cadherin-11 and TNF-¦Á, and HE staining was used to detect the pathological changes in the synovial tissue. Results The synovial tissue of the model mice showed similar characteristics to the pathological process of rheumatoid arthritis. The inflammatory response of the synovial tissue of the model mice treated with lentivirus with high expression of miRNA-144 was compared with that of the saline and empty lentivirus treated groups. The expression of TNF-¦Á was significantly reduced compared with the normal saline and empty vector lentivirus treatment groups (P<0.05). Further detection of the expression of Cadherin-11 was also significantly reduced (P<0.05). Conclusion Increased miRNA-144 in the body can attenuate or inhibit the inflammatory response of synovial tissue in arthritis model mice and downregulate the expression of TNF-¦Á, and this effect may be due to the inhibition of Cadherin-11 in synovial cells by miRNA-144. effect. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yan Yanfei, Kang Fei, Yang Bo, Miao Ying, Dong Shiwu
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
