Effects Of Prunella Vulgaris Sulfated Polysaccharide On Rat Liver Fibrosis Induced By Ccl4 And Activation Of Rat Hepatic Stellate Cells Induced By Tgf-¦¢1
Keywords
Prunella Vulgaris Sulfated Polysaccharide Liver Fibrosis Hepatic Stellate Cells Type I Collagen ¦¡-Smooth Muscle Actin
Abstract
Objective: To observe the effects of Prunella vulgaris sulfated polysaccharide (PSSP) on rat liver fibrosis induced by carbon tetrachloride (CCl4) and transforming growth factor-¦Â1 (TGF-¦Â1)-induced activation of rat hepatic stellate cells-T6 (HSC- T6), thus preliminarily exploring the anti-fibrosis mechanism of PSSP. Methods: Liver fibrosis modeling was induced in rats by intraperitoneal injection of CCl4-olive oil solution, and they were randomly divided into 5 groups, namely normal group, model group, colchicine group (0.25 mg¡¤kg-1), and PSSP high-dose group. (300 mg¡¤kg-1) and PSSP low-dose group (100 mg¡¤kg-1). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), laminin (LN), hyaluronic acid (HA), type III procollagen (PCIII) and The content of collagen IV (C-IV); hematoxylin-eosin (HE) and Sirius red staining were used to observe the pathological changes of rat liver tissue; HSC-T6 cells were cultured in vitro; the cell proliferation and activity method (CCK-8) was used The effect of PSSP on the proliferation of HSC-T6 cells induced by TGF-¦Â1 was determined; real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) and Western blot were used to detect type ¢ñ collagen ( Col-I), ¦Á-smooth muscle actin (¦Á-SMA) mRNA and protein expression. Results: Compared with the model group, PSSP could significantly reduce the levels of ALT, AST, HA, LN, PC-¢ó and C-¢ô in rats, and reduce the degree of liver fibrosis in rats (P<0.01); PSSP could inhibit TGF- The proliferation of HSC-T6 cells induced by ¦Â1 reduced the relative expression of Col-I and ¦Á-SMA mRNA and protein in HSC-T6 cells (P<0.01). Conclusion: PSSP has a good anti-fibrosis effect, and its mechanism may be related to inhibiting the activation of hepatic stellate cells, inhibiting collagen synthesis and deposition, reducing the production of extracellular matrix (ECM) and promoting its degradation. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Fu Yueyue, Zhang Shuangxia, Zhang Guoliang, Zhu Lanping
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
