Effects Of Salvianolic Acid B Salt On Myocardial Basement Membrane Type Collagenase Activity And Its Regulatory Factors In Rats With Cardiac Hypertrophy
Keywords
Salvianolic Acid B Salt, Myocardial Fibrosis, Type Iv Collagen, Basement Membrane Matrix Metalloproteinase-2/9, Activity
Abstract
Objective: To explore the anti-myocardial fibrosis mechanism of salvianolic acid B salt (SA-B) on basement membrane-type matrix metalloproteinases and their inhibitors in fibrotic hearts. Methods: Rat cardiac hypertrophy model was prepared by incomplete ligation of the abdominal aorta. Rats were randomly divided into sham operation group, model group, salvianolic acid B salt low, medium and high dose groups (10, 20, 40mg¡¤kg-1¡¤d-1) and captopril group (40mg¡¤kg- 1¡¤d-1). HE and Sirius red collagen staining were used to observe left ventricular tissue pathology and collagen deposition changes; Western blotting was used to analyze type IV collagen, MMP-2/9 and TIMP-2 protein expression in myocardial tissue; gelatin zymography was used to determine MMP-2/9 activity. . Results: Compared with the model group, the SA-B intervention group had reduced myocardial inflammation, reduced collagen deposition, significantly reduced Hyp content in myocardial tissue, and significantly reduced the degree of fibrosis; type IV collagen, MMP-2/9, TIMP- 2 protein expression and MMP-2 activity were significantly increased. The SA-B intervention group reduced type IV collagen, MMP-2/9 and TIMP-2 protein expression, and reduced MMP-2 activity. The effect was more obvious in the medium and high dose groups. Conclusion: One of the mechanisms of SA-B’s anti-myocardial fibrosis action is to inhibit the expression of TIMP-2, reduce the expression and activity of basement membrane collagenase, and reduce the damage of normal basement membrane collagen.
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Original research was done by Wu Qi, Peng Yuan, Meng Qianchao, Cui Hongyan, Wang Xiaoning
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