Immunological Response Evaluation Of Absorbable Collagen Membrane In Vivo
Keywords
Collagen Membrane, Bone Repair, Immune Response, Flow Cytometry, Inflammation Collagen Membrane, Bone Regeneration, Immune Response, Flow Cytometry, Inflammation
Abstract
Abstract Immune response has always been one of the key factors limiting the application of implantable biomaterials. The experiment evaluated the immune response of two types of collagen membranes used for bone repair in the body, hoping to provide a basis for their clinical trials. On the 14th day after the two membranes were subcutaneously implanted into BALB/c mice, compared with the negative control (NC, no implanted material), the spleen and lymph nodes were not significantly enlarged, and there was almost no difference in the number of lymph node cells. The number of spleen cells is approximately 2 times that of NC. Flow cytometry analysis showed that implantation of collagen membrane 1 led to a reduction of approximately 13% in the proportion of T cells in the spleen, but did not affect T cell subsets, while implantation of collagen membrane 2 had no significant effect on the composition of spleen cells in mice; 2 species Collagen membranes activated a certain amount of B cells, and the activation rate was approximately twice that of NC mice. The lymphocyte proliferation experiment in vitro showed no significant difference from the NC group. Enzyme-linked immunosorbent assay showed that collagen membrane 1 caused the antibody concentration in serum to increase to 2 times that of NC mice on day 14. Local H&E staining showed that both materials caused slight cellular infiltration. The immune responses caused by these two collagen membranes are very weak and can be used in clinical trials. Abstract: Immune response is one of the critical factors to limit implantable biomaterials’ application. In this study, two kinds of collagen membranes for bone regeneration were evaluated in terms of immunological response in vivo, which is hoped to lay foundations for clinical trials. At day 14 following subcutaneous implantation of the two membranes in BALB/c mice, compared with negative control (NC, no implant), spleen and lymph nodes showed no obvious swelling, and normal lymph nodes’cell population as well as one-fold more splenic cell population was observed. Flow cytometry analysis demonstrated that in mice with collagen membrane 1, splenic T lymphocytes percentage decreased by about 13%, but no significant change was discovered in T lymphocyte subsets; collagen membrane 2 did not trigger apparent splenic cells composition alteration; the two membranes activated one-fold more B lymphocytes than NC. Enzyme linked immunosorbent assay indicated that one-fold higher IgG concentration than NC was detected in mice with collagen membrane 1 in day 14. Lymphocytes proliferation assay in vitro did not show significant difference from NC. H&E staining of local tissues exhibited slight cell infiltration around the two membranes. The two collagen membranes have engendered mild immune response and can be applied to clinical trials.
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Original research was done by Zhang Lin, Sun Lei, Xu Mengjun, Niu Xufeng
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