Inhibitory Effect Of Cordyceps Militaris On The Progression Of Renal Disease In Diabetic Rats
Keywords
Connective Tissue Growth Factor, Transforming Growth Factor-¦¢1, Type Iv Collagen, Cordyceps Militaris, Diabetic Nephropathy
Abstract
Objective: To study the inhibitory effect of Cordyceps militaris on the development of renal disease in diabetic rats. Methods: A diabetic rat model was established using a one-time intraperitoneal injection of streptozotocin (STZ). After oral administration of Cordyceps militaris (400 mg/kg, based on body weight) for 8 weeks, the fasting blood glucose levels and renal index of the rats were measured respectively; 24-hour urine of the rats was collected to detect trace albumin (mAlb) and ¦Â in the urine. -N-acetylglucosidase (NAG) content; determination of urea nitrogen (BUN), creatinine (Cr), and uric acid (UA) contents in serum; determination of transforming growth factor ¦Â1 (TGF-¦Â1) and its contents in kidney by RT-PCR method Type II receptor (T¦ÂR¢ò) and connective tissue growth factor (CTGF) mRNA expression; Western blotting to detect TGF-¦Â1 protein expression in the kidney; immunohistochemistry to detect type IV collagen expression in the kidney. Results: Cordyceps militaris can significantly reduce fasting blood glucose (P£¼0.05), renal index (P£¼0.01), 24h urine output (P£¼0.01), urinary microalbumin excretion rate (UAER) (P£¼0.01) and NAG excretion rate (P<0.01); at the same time, it can reduce the levels of BUN, Cr, and UA in serum (P<0.05); down-regulate the mRNA expression of TGF-¦Â1, TGF¦ÂR¢ò and CTGF (P<0.01), and reduce TGF-¦Â1 and type IV Collagen protein expression (P<0.01). Conclusion: Cordyceps militaris has good effects on lowering blood sugar and improving kidney filtration function. Its mechanism may be related to inhibiting the expression of genes such as TGF-¦Â1, TGF¦ÂR¢ò and CTGF, and reducing the accumulation of collagen substances in the kidneys. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Li Bing, Li Xiaolin, Yang Yancun, Yang Yuhong, Teng Laibin, Wang Jingfeng, Xue Changhu
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