Omentin Improves The Inhibitory Effect Of Oxidative Stress On The Expression Of Type ¢ñ And ¢ô Collagen In Human Arterial Vascular Smooth Muscle Cells
Keywords
Omentin Human Aortic Smooth Muscle Cells Oxidative Stress Type ¢ñ Collagen Type ¢ô Collagen
Abstract
Objective To study the effect of omentin on the expression of collagen ¢ñ and ¢ô in human aortic smooth muscle cells under oxidative stress. Methods Human aortic smooth muscle cell lines were cultured. After the cell density reached more than 90%, they were randomly divided into 5 groups: control group, oxidative stress group, omentin group, extracellular regulated protein kinase (ERK) inhibitor group and silk Mitogen-activated protein kinase (p38MAPK) inhibitor group. Among them, the control group did not add any treatment, the oxidative stress group added tert-butyl hydroperoxide (t-BHP, 87.1 ¦Ìmol/L), and the omentin group (87.1 ¦Ìmol/L t-BHP+600 ¦Ìg/L omentin) ), ERK inhibitor group and p38MAPK inhibitor group were added with ERK inhibitor (PD98059, 60 ¦Ìmol/L) and p38MAPK inhibitor (SB203580, 100 ¦Ìmol/L) respectively on the basis of omentin group, and were detected by Western blot method. Expression of collagen I and IV in cells. Results Using the MTT method, the optimal concentration of t-BHP was screened to be 87.1 ¦Ìmol/L; compared with the control group, the expression of type ¢ñ and ¢ô collagen in human arterial smooth muscle cells in the oxidative stress group significantly decreased (P<0.01); compared with the oxidative stress group Compared with the stress group, the expression of type ¢ñ and ¢ô collagen in the omentin group was significantly increased (P<0.01); compared with the omentin group, the expression of type ¢ñ and ¢ô collagen in the ERK inhibitor group and p38MAPK inhibitor group was significantly decreased (P<0.01). P<0.05). Conclusion Omentin can improve the inhibitory effect of oxidative stress on the expression of type I and IV collagen in human arterial smooth muscle cells, and may promote the stability of atherosclerotic plaques through this mechanism. The ERK/p38MAPK pathway may be involved in omentin signaling. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Huijuan, Zhang Chunfeng, Li Xuefen, Hu Rui, Ge Huanqi, Zheng Hui
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