Optimization Of The Process For Extracting Collagen From Salamander Skin Using High-Density Co2 Treatment
Keywords
Salamander Skin, High-Density Co2, Response Surface Test, Collagen Molecular Structure, Extraction Rate
Abstract
In order to study the effect of high-density CO2 (dense phase carbon dioxide, DPCD) pre-treatment on the extraction rate of collagen from salamander skin, freeze-dried salamander skin was used as raw material, DPCD was used to pre-treat salamander skin, and collagen from salamander skin was extracted by enzymatic method. A single factor experiment and a response surface experiment were designed to optimize the process conditions of DPCD treatment of salamander skin using the collagen extraction rate as an evaluation index. The results show that the optimal temperature for DPCD treatment of salamander skin is 32¡ãC, pressure 30 MPa, and time 6 h; under these processing conditions, the model predicts that the extraction rate of collagen from salamander skin is 39.70%, and the verification value is (39.48¡À0.74)% , there is no significant difference (P>0.05); compared with the collagen extraction rate of the raw material without DPCD treatment ((20.63¡À0.46)%), the extraction rate increased by 18.85%; the microstructure shows that the salamander skin after DPCD treatment The tissue is loose, the regular arrangement of micellar fibers is destroyed, and it appears porous; the electrophoresis pattern shows that after salamander skin is treated with DPCD, the collagen maintains the typical structural characteristics of type I collagen. DPCD treatment of salamander skin significantly improves the collagen extraction rate while maintaining the structural characteristics of collagen in salamander skin.
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Original research was done by Ren Guoyan, Feng Qiuqi, Song Ya, Cui Guoting, Kang Huaibin, Wang Songjun, Xiao Feng, Guo Jinying
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