Oxymatrine Inhibits Tgf-¦¢1-Induced Cfbs Proliferation By Downregulating P38Mapk Phosphorylation And Improving Collagen Deposition.
Keywords
Oxymatrine, Tgf-¦¢1, Cardiac Fibrosis, Cardiac Fibroblasts, Collagen, P38Mapk
Abstract
Objective: To study the effect of oxymatrine (OMT) on TGF-¦Â1-induced proliferation of cardiac fibroblasts (CFBs) and analyze the possible mechanism of action. Methods: The experiment was divided into blank control group (serum-free DMEM), model group (20 ¦Ìg¡¤L-1 TGF-¦Â1), OMT low-dose group (OMT-L group, 1.89¡Á10-4mol¡¤L-1), and OMT medium-dose group. group (OMT-M group, 3.78¡Á10-4mol¡¤L-1), OMT high-dose group (OMT-H group, 7.56¡Á10-4mol¡¤L-1) and SB203580 (p38MAPK blocker, 1¡Á10- 5mol¡¤L-1). Immunocytochemistry was used to identify vimentin in CFBs, and determine the expression of ¦Á-SMA after TGF-¦Â1 induced CFBs for 24 hours; MTT method was used to analyze the inhibitory effect of CFBs proliferation; picric acid Sirius red staining was used to observe the deposition of type ¢ñ collagen and type ¢ó collagen; Westernblot analysis of the expression of p38MAPK, p-p38MAPK, type ¢ñ collagen and type ¢ó collagen. Results: MTT results showed that 3.78¡Á10-4 and 7.56¡Á10-4mol¡¤L-1 OMT had an inhibitory effect on the abnormal proliferation of CFBs induced by TGF-¦Â1 (P<0.01); ¦Á-SMA immunocytochemistry experiments showed that OMT could Inhibits CFBs-myFBs conversion; Picric acid Sirius red staining shows that OMT can significantly reduce the deposition of type I and type III collagen; Westernblot shows that OMT can significantly inhibit TGF-¦Â1-induced deposition of type I and type III collagen and the phosphorylation of p38MAPK (P<0.01). Conclusion: OMT can inhibit the proliferation of CFBs induced by TGF-¦Â1 and inhibit the expression of related collagen, improving myocardial fibrosis. The mechanism may be related to the inhibition of p38MAPK phosphorylation. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Xiao Hai, Xu Yini, Luo Hong, Chen Yan, Zhang Yanyan, Tao Ling, Jiang Yan, Shen Xiangchun
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