Prokaryotic Expression And Functional Study Of A Novel Recombinant Human-Derived Collagen Prokaryotic Expression And Functional Study Of A Novel Recombinant Human-Derived Collagen That Promotes Cell Adhesion
Keywords
Recombinant Human-Derived Collagen Cell Adhesive Prokaryotic Expression Functional Study Recombinant Human-Derived Collagen, Cell Adhesion, Prokaryotic Expression, Functional Study
Abstract
The DNA sequences, which originated from gene COL1A1, optimized for E.coli codon usage and contained cell adhesion domains of GER units, was amplified by RT-PCR technology from human tissue. The recombinant expression vector pET28a( )-COL encoded the recombinant human -derived collagen (RHDC) was constructed and transformed host E.coli strain BL21(DE3)pLysS. The RHDC production was induced by the addition of IPTG and approximately 40% of the total strain proteins. RHDC could form a triple-helical structure suggested by the SDS-PAGE and Western blot analysis. Cell adhesion assay indicated that RHDC had high cell adhesive character, which makes it suitable for use as biomaterials.
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Original research was done by Kun Hu Fu-zhai Cui Li Xu Heng-liang Wang Liu-yu Huang, Hu Kun, Cui Fuzhai, Xu Li, Wang Hengliang, Huang Liuyu
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