Study On The Effect Of Pollock Skin Collagen Peptide On Bone Microstructure In Ovariectomized Rats
Keywords
Pollock Skin Collagen Peptide, Osteoporosis, Calcitonin Receptor, Il-1, Bone Microstructure, Rat
Abstract
Objective To explore the feasibility of preventing and treating osteoporosis by observing the effect of pollock skin collagen peptide on the bone microstructure of osteoporosis model in ovariectomized rats. Methods 60 adult Wistar female rats, body weight (250¡À10) g, were randomly divided into 5 groups (n=12), namely normal control group (Group A), osteoporosis model group (Group B), bone Osteoporosis model + pollock skin collagen peptide prevention group (Group C), osteoporosis model + pollock skin collagen peptide low-dose treatment group (Group D), osteoporosis model + pollock skin collagen peptide high-dose treatment group (Group D) Group E), 12 animals per group. Groups B, C, D, and E used bilateral ovary removal to prepare rat osteoporosis models. Starting from 4 weeks before surgery, Group C and Groups D and E were given pollock skin collagen peptide intragastrically at 1.0, 0.5 and 1.0 g/kg every day for 6 consecutive weeks starting from 6 weeks after surgery; At the same time point, an equal volume of normal saline was administered by gavage. 24 hours after the last administration, X-ray films of the femurs of rats in groups A and B were taken and the gray value was measured; rats in each group were then sacrificed by cervical dislocation, and the left proximal tibia bone tissue was taken for HE staining to observe bone tissue disease. Physical changes were measured, including trabecular number (TN), mean trabecular plate thickness (MTPT), mean trabecular plate spacing (MTPS), and trabecular volume percentage (trabecular bone volume (TBV), mean bone cortical thickness (MBCT); immunohistochemical staining to observe calcitonin receptor (caltitonin receptor (CTR)) and IL-1 expression levels. Results The femoral gray value of rats in group B was significantly lower than that of group A (t=45.130, P=0.000), indicating that the osteoporosis model of ovariectomized rats was successfully prepared. Histological observation showed that there were statistically significant differences in TN, MTPS, TBV, and MBCT in groups A, C, and E compared with group B (P<0.05); compared with groups D and E, the morphometric parameters of each bone tissue in group C were The differences were all statistically significant (P<0.05); the differences in TN, MTPS, TBV, and MBCT in group D were statistically significant compared with group A (P<0.05); in group E, only the differences in MTPS and group A were statistically significant ( P<0.05); compared with group D, the differences in MTPS, TBV and MBCT of group E were statistically significant (P<0.05). Immunohistochemistry staining observation showed that the expression levels of CTR and IL-1 in groups A, C, D, and E were lower than those in group B, and those in groups C and E were lower than those in group D, with statistically significant differences (P<0.05); among the other groups The differences were not statistically significant (P>0.05). Conclusion Pollock skin collagen peptide can improve the bone microstructure of osteoporotic rats, and its mechanism may be related to inhibiting the expression of CTR and IL-1 in bone tissue. However, it has not been found to have a preventive effect on osteoporosis.
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Original research was done by Wu Binbin, Zhang Yimeng, Yang Jieru, Wang Chunbo, Xing Lifeng, Jin Lixin, Han Yantao
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